Aliquot and store in the dark at 2°C to 8°C. Avoid exposure to light.
Reactivity
Human
Note
This product is for research use only.
Clonality
monoclonal
Buffer
The reagent is provided in aqueous buffered solution containing protein stabilizer, and 0.09% sodium Azide
Recommended Dilution
FCM: 1 µg/1 million cells. Optimal dilutions/concentrations should be determined by the end user.
Description
Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies. The evaluation of cell surface Kappa/Lambda expression can identify clonally restricted B lymphocyte populations and thus can aide in the diagnosis of hematologic malignancy. Several B cell disorders are associated with decreased levels of Kappa/Lambda at the cell surface.