Viral Tools for Neural Circuit Tracing

The brain neural network is a complex structure composed of a large number of neurons with different shapes and characteristics through synaptic connections, which is the structural basis for the brain to exercise cognitive, emotional, memory, imagination and other activities. Mapping neuronal projections can track the flow of information between different brain regions.

For years, traditional non-viral methods have been widely used to trace neural circuits. These include injection of chemicals like phytohemagglutinin or horseradish peroxidase (HRP) and biocytin into specific brain regions to track fibrous connections of peripheral nerves; radiolabeled amino acids that are absorbed and incorporated into the proteins of neurons; dye injection; electrophysiological methods. However, most conventional tracers have limited specificity, low efficiency, lack of temporal control, and high risk of damaging surrounding tissues in highly invasive surgeries.

​Instead, viral tools represent a significant advancement in neural circuit tracing, offering a more refined and targeted approach. These engineered viral vectors are designed to deliver genetic material to neurons, allowing for precise labeling, monitoring, and manipulation of neural circuits.

Biohippo offers a variety of viral vectors, including RV, HSV, PRV, and VSV, to replace conventional tracers with the highly efficient, more specific, and less invasive viral method.