Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.
Purification
Unpurified ascites.
Isotype
IgG
Tested Application
ELISA, WB, IHC
Gene Id
4961474
Availability
Shipped within 5-10 working days.
Target
KSHV ORF45
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity
Virus
Note
This product is for research use only.
Clonality
monoclonal
Buffer
Ascitic fluid containing 0.03% sodium azide.
Recommended Dilution
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Description
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma- (2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease.The genome of KSHV is 165-170 kb and contains at least 88 open reading frames.ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells.Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7.In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN.Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions.
Immunocytochemistry analysis of TPA induced BCBL-1 cells (A) and uninduced BCBL-1 cells (B) using KSHV ORF45 antibody with AEC staining.