Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Isotype
IgG
Tested Application
P-ELISA, WB, IF/ICC, FCM
Gene Id
3607
Availability
Shipped within 1-3 working days.
Concentration
0.5 mg/ml
Target
ILF (Isoform 1)
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity
Human
Note
This product is for research use only.
Clonality
polyclonal
Buffer
Tris saline, pH 7.3, containing 0.02% sodium azide and 0.5% bovine serum albumin.
Description
ILF (Isoform 1) Antibody is a Goat Polyclonal antibody against ILF (Isoform 1).
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ?g/ml) followed by Alexa Fluor 488 secondary antibody (2 ?g/ml), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ?g/ml) followed by Alexa Fluor 488 secondary antibody (2 ?g/ml).
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ?g/ml) followed by Alexa Fluor 488 secondary antibody (2 ?g/ml), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ?g/ml) followed by Alexa Fluor 488 secondary antibody (2 ?g/ml).
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ?g/ml) followed by Alexa Fluor 488 secondary antibody (1 ?g/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.