Purified recombinant fragment of human C-CBL expressed in E. Coli.
Purification
Unpurified ascites.
Isotype
IgG
Tested Application
ELISA, WB, IHC, IF/ICC, FCM
Uniprotkb
P22681 (UniProt, ExPASy)
Gene Id
867
Availability
Shipped within 5-10 working days.
Target
C-CBL
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity
Human, Mouse, Rat
Molecular Weight
120 kDa
uniprot enrty name
CBL_HUMAN
Note
This product is for research use only.
Clonality
monoclonal
Buffer
Ascitic fluid containing 0.03% sodium azide.
Recommended Dilution
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, IF/ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user.
Description
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation.
Western blot analysis using C-CBL antibody against RAJI (1), RAW264.7 (2), K562 (3), SKBR-3 (4), 3T3-L1 (5), THP-1 (6) and PC-12 (7) cell lysate.
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using C-CBL antibody with DAB staining.
Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C-CBL antibody with DAB staining.
Immunofluorescence analysis of Hela cells using C-CBL antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.
Flow cytometric analysis of MCF-7 cells using C-CBL antibody (blue) and negative control (red).