Mitogen-Activated Protein Kinase 3 Phospho-Tyr204 (ERK1/2 pY204) Antibody

Mfr.No.	abx225435
Conjugate	Unconjugated
Gene Symbol	MAPK1, MAPK3
String	9606.ENSP00000361423 9606.ENSP0000042756 10090.ENSMUSP00000075167 10090.ENSMUSP00000027888
Host	Rabbit
Form	Liquid
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ERK1/2 (pY204). The exact sequence is proprietary.
Purification	The antibody was purified by immunogen affinity chromatography.
Tested Application	WB, IHC, IF/ICC
Uniprotkb	P27361 (UniProt, ExPASy) P28482 (UniProt, ExPASy) Q63844 (UniProt, ExPASy) P63085 (UniProt, ExPASy) P21708 (UniProt, ExPASy) P63086 (UniProt, ExPASy)
Gene Id	5595 5594 26417 26413 50689 116590
Availability	Shipped within 5-10 working days.
Target	Mitogen-Activated Protein Kinase 3 Phospho-Tyr204 (ERK1/2 pY204)
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity	Human, Mouse, Rat, Cow, Chicken, Zebrafish
uniprot enrty name	MK03_HUMAN, MK01_HUMAN, MK03_MOUSE, MK01_MOUSE, MK03_RAT, MK01_RAT
Note	This product is for research use only.
Clonality	polyclonal
Buffer	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Recommended Dilution	WB: 1/500 - 1/1000, IHC: 1/50 - 1/200, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Description	Mitogen-Activated Protein Kinase 3 Phospho-Tyr204 (ERK1/2 pY204) Antibody is a Rabbit Polyclonal against Mitogen-Activated Protein Kinase 3 Phospho-Tyr204 (ERK1/2 pY204).

Immunohistochemical analysis of ERK1/2 (pY204) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of ERK1/2 (pY204) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ?C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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SKU	Supplier No.	Size	Your price	Quantity
BHA14469267	abx225435	30 ul	$217.50		Add to Cart
BHA14469267	abx225435	100 ul	$304.50		Add to Cart

Mitogen-Activated Protein Kinase 3 Phospho-Tyr204 (ERK1/2 pY204) Antibody

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