Purified recombinant fragment of human TIP60 expressed in E. Coli.
Purification
Unpurified ascites.
Isotype
IgG
Tested Application
ELISA, WB, IHC
Uniprotkb
Q92993 (UniProt, ExPASy)
Gene Id
10524
Availability
Shipped within 5-10 working days.
Target
TIP60
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity
Human
Molecular Weight
60 kDa
uniprot enrty name
KAT5_HUMAN
Note
This product is for research use only.
Clonality
monoclonal
Buffer
Ascitic fluid containing 0.03% sodium azide.
Recommended Dilution
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Description
HTATIP (HIV-1 Tat interacting protein TIP60, about 60kDa) belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.
Immunohistochemical analysis of paraffin-embedded human liver carcinoma (A), recturn carcinoma (B), normal medulla tissue (C) and normal interbrain tissues (D), showing nuclear localization using Tip60 antibody with DAB staining.
Reviews of Histone Acetyltransferase Tip60 (TIP60) Antibody