Purified recombinant fragment of CK1 expressed in E. Coli.
Purification
Unpurified ascites.
Isotype
IgG
Tested Application
ELISA, WB, IHC
Uniprotkb
P48729 (UniProt, ExPASy)
Gene Id
3848
Availability
Shipped within 5-10 working days.
Target
CK1
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Reactivity
Human
Molecular Weight
39 kDa
Note
This product is for research use only.
Clonality
monoclonal
Buffer
Ascitic fluid containing 0.03% sodium azide.
Recommended Dilution
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Description
CK1 (also designated Cytokeratin 1 or KRT1),with 644-amino acid protein (about 70kDa), is a member of the keratin family. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells.CK1 consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK1 is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.
Immunohistochemical analysis of paraffin-embedded human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization using CK1 antibody with DAB staining.
CK1 Antibody
Immunohistochemical analysis of paraffin-embedded human liver tissue (A), colon carcinoma (B), lung carcinoma (C) and esophagus tissue (D), showing membrane localization using CK1 antibody with DAB staining.