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Stable Cell Line Generation
Stable Cell Line Generation
It is common to use stable cell lines for gene function (protein function), recombinant protein and antibody production, disease modeling, drug discovery, and many other applications. Among our services are gene expression stable cell lines, gene suppression stable cell lines (knock-downs and knock-outs), and genome editing stable cell lines using eukaryotic expression vectors or integrated lentiviral vectors.
Our advantages for stable cell line generation
● The largest collection of expression-ready ORF clones and lentiviral particles reduces turnaround time and ensures high quality.
● A wide variety of cell backgrounds ranging from HEK293 to iPSCs.
● An expert in CRISPR technology.
● A full-service provider for stable cell line generation projects.
Stable Cell Line Service Details
Gene expression stable cell line generation
● Construct vector
Construct your gene of interest into a suitable expression vector (a eukaryotic expression vector or an integrated lentiviral vector).
● Transient expression
Perform transfection or transduction on target cells.
• Stable cell clones selection
Resistance to antibiotics or flow cytometry screen stable cell clones.
• Gene expression evaluation
Evaluate the gene expression in stable cell clones via one or several assays such as Western Blot, ELISA, real-time PCR, or reporter assays, etc.
Gene Knockdown stable cell line generation
• shRNA construction
Construct shRNA targeting gene of interest into a suitable expression vector (an eukaryotic expression vector or an integrated lentiviral vector).
● Screening of shRNA efficacy
● Transient expression
Perform transfection or transduction on target cells.
• Stable cell clones selection
Resistance to antibiotics or flow cytometry screen stable cell clones.
• Validation
Validation RNAi gene silencing by real-time qRT-PCR and/or Western blot.
Gene Knock-in stable cell line generation
● gRNA/Donor construction
● Validation of cas9- gRNA efficacy
● Perform transfection or transduction on target cells
● Screening of positive cell clones
● Quality control analysis of stable cell line
Gene Knockout stable cell line generation
● gRNA construction
● Validation of cas9- gRNA efficacy
● Perform transfection or transduction on target cells
● Screening of positive cell clones
● Quality control analysis of stable cell line
If you have any needs, just email us at [email protected].
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