Anti-Zebrafish OPA1 Antibody Picoband®

Mfr.No.	AZQ5U3A7
Background	Dynamin-like 120 kDa protein, mitochondrial is a protein that in humans is encoded by the OPA1 gene. It is mapped to 3q29. This protein regulates mitochondrial fusion and cristae structure in the inner mitochondrial membrane (IMM) and contributes to ATP synthesis and apoptosis. This gene product is a nuclear-encoded mitochondrial protein with similarity to dynamin-related GTPases. It is a component of the mitochondrial network. Mutations in this gene have been associated with optic atrophy type 1, which is a dominantly inherited optic neuropathy resulting in progressive loss of visual acuity, leading in many cases to legal blindness. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish OPA1 Antibody Picoband® catalog #AZQ5U3A7. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish OPA1 recombinant protein (Position: D302-Q568)
Molecular Weight	112 kDa
Positive Control	WB: whole female zebrafish tissue, whole male zebrafish tissue IHC: zebrafish brain tissue, zebrafish eye tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Dynamin-like 120 kDa protein, mitochondrial
Gene Name	OPA1
Weight	112 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ5U3A7 is guaranteed for IHC, WB Boster Guarantee

Western blot analysis of OPA1 using anti-OPA1 antibody (AZQ5U3A7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: whole female zebrafish tissue lysates,Lane 2: whole male zebrafish tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPA1 antigen affinity purified polyclonal antibody (AZQ5U3A7) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for OPA1 at approximately

IHC analysis of OPA1 using anti-OPA1 antibody (AZQ5U3A7). OPA1 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-OPA1 Antibody (AZQ5U3A7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of OPA1 using anti-OPA1 antibody (AZQ5U3A7). OPA1 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-OPA1 Antibody (AZQ5U3A7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011304-1	AZQ5U3A7	Unconjugated	100 ug	$470	Add to Cart
BHA21011304-2	AZQ5U3A7-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011304-3	AZQ5U3A7-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011304-4	AZQ5U3A7-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011304-5	AZQ5U3A7-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011304-6	AZQ5U3A7-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011304-7	AZQ5U3A7-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011304-8	AZQ5U3A7-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011304-9	AZQ5U3A7-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011304-10	AZQ5U3A7-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011304-11	AZQ5U3A7-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011304-12	AZQ5U3A7-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011304-13	AZQ5U3A7-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish OPA1 Antibody PicobandÂ®

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