Anti-Zebrafish NOVA2 Antibody Picoband®

Mfr.No.	AZF1R4G7
Background	NOVA alteative splicing regulator 2 is a protein that in humans is encoded by the NOVA2 gene. It is mapped to 19q13.32. NOVA2 may regulate RNA splicing or metabolism in a specific subset of developing neurons. It binds single strand RNA.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish NOVA2 Antibody Picoband® catalog #AZF1R4G7. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish NOVA2 recombinant protein (Position: M25-Q229)
Molecular Weight	49 kDa
Positive Control	WB: zebrafish head tissue, zebrafish embryo tissue IHC: zebrafish brain tissue, zebrafish heart tissue, zebrafish eye tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	RNA-binding protein Nova-2
Gene Name	NOVA2
Weight	49 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZF1R4G7 is guaranteed for IHC, WB Boster Guarantee

Western blot analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOVA2 antigen affinity purified polyclonal antibody (AZF1R4G7) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for NOVA2 at approximately 55 kDa.

IHC analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). NOVA2 was detected in a paraffin-embedded section of zebrafish eye tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-NOVA2 Antibody (AZF1R4G7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). NOVA2 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-NOVA2 Antibody (AZF1R4G7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of NOVA2 using anti-NOVA2 antibody (AZF1R4G7). NOVA2 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-NOVA2 Antibody (AZF1R4G7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011301-1	AZF1R4G7	Unconjugated	100 ug	$470	Add to Cart
BHA21011301-2	AZF1R4G7-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011301-3	AZF1R4G7-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011301-4	AZF1R4G7-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011301-5	AZF1R4G7-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011301-6	AZF1R4G7-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011301-7	AZF1R4G7-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011301-8	AZF1R4G7-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011301-9	AZF1R4G7-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011301-10	AZF1R4G7-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011301-11	AZF1R4G7-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011301-12	AZF1R4G7-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011301-13	AZF1R4G7-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish NOVA2 Antibody PicobandÂ®

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