Anti-Zebrafish NOL10 Antibody

Mfr.No.	AZQ802W4
Background	By immunoprecipitation of human AATF we discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10, and demonstrate that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish NOL10 Antibody catalog #AZQ802W4. Tested in IHC applications. This antibody reacts with Zebrafish.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish NOL10 recombinant protein (Position: Y73-D497)
Positive Control	IHC: zebrafish kidney tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Nucleolar protein 10
Gene Name	NOL10
Weight	80.302kDa

Dilutions	Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC
Description	AZQ802W4 is guaranteed for IHC Boster Guarantee

IHC analysis of NOL10 using anti-NOL10 antibody (AZQ802W4). NOL10 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-NOL10 Antibody (AZQ802W4) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011299-1	AZQ802W4	Unconjugated	100 ug	$470	Add to Cart
BHA21011299-2	AZQ802W4-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011299-3	AZQ802W4-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011299-4	AZQ802W4-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011299-5	AZQ802W4-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011299-6	AZQ802W4-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011299-7	AZQ802W4-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011299-8	AZQ802W4-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011299-9	AZQ802W4-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011299-10	AZQ802W4-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011299-11	AZQ802W4-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011299-12	AZQ802W4-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011299-13	AZQ802W4-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish NOL10 Antibody

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