Anti-Zebrafish METAP2a/b Antibody Picoband®

Mfr.No.	AZA5WVX8
Background	Methionine aminopeptidase 2 is an enzyme that in humans is encoded by the METAP2 gene. The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish METAP2a/b Antibody Picoband® catalog #AZA5WVX8. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish METAP2a/b recombinant protein (Position: E191-R464)
Molecular Weight	53 kDa
Positive Control	WB: zebrafish embryo tissue IHC: zebrafish brain tissue, zebrafish spinal cord tissue, zebrafish heart tissue, zebrafish muscle tissue, zebrafish kidney tissue, zebrafish liver tissue, zebrafish colon tissue, zebrafish pancreas tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Gene Name	METAP2a/b
Weight	53 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZA5WVX8 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +5 METAP2a/b was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-ME

Western blot analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-METAP2a/b antigen affinity purified polyclonal antibody (AZA5WVX8) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for METAP2a/b at approximately 67 kDa. The expected band siz

IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of METAP2a/b using anti-METAP2a/b antibody (AZA5WVX8). METAP2a/b was detected in a paraffin-embedded section of zebrafish spinal cord tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-METAP2a/b Antibody (AZA5WVX8) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011311-1	AZA5WVX8	Unconjugated	100 ug	$470	Add to Cart
BHA21011311-2	AZA5WVX8-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011311-3	AZA5WVX8-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011311-4	AZA5WVX8-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011311-5	AZA5WVX8-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011311-6	AZA5WVX8-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011311-7	AZA5WVX8-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011311-8	AZA5WVX8-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011311-9	AZA5WVX8-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011311-10	AZA5WVX8-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011311-11	AZA5WVX8-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011311-12	AZA5WVX8-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011311-13	AZA5WVX8-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish METAP2a/b Antibody PicobandÂ®

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