Anti-Zebrafish Lysozyme/LYZ Antibody Picoband®

Mfr.No.	AZQ90YS5
Background	In humans, the lysozyme enzyme is encoded by the LYZ gene. This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta (1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish Lysozyme/LYZ Antibody Picoband® catalog #AZQ90YS5. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish Lysozyme/LYZ recombinant protein (Position: K19-A151)
Molecular Weight	17 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish liver tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Lysozyme C
Gene Name	LYZ
Weight	17 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ90YS5 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (AZQ90YS5). Lysozyme/LYZ was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-Lysozyme/LYZ Antibody (AZQ90YS5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (AZQ90YS5). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates.Lane 2: whole female zebrafish tissue lysates.Lane 3: whole male zebrafish tissue lysates.Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lysozyme/LYZ antigen affinity purified polyclonal antibody (AZQ90YS5) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate

IHC analysis of Lysozyme/LYZ using anti-Lysozyme/LYZ antibody (AZQ90YS5). Lysozyme/LYZ was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-Lysozyme/LYZ Antibody (AZQ90YS5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Have you used this product?

Rate it now..!!

Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011370-1	AZQ90YS5	Unconjugated	100 ug	$470	Add to Cart
BHA21011370-2	AZQ90YS5-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011370-3	AZQ90YS5-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011370-4	AZQ90YS5-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011370-5	AZQ90YS5-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011370-6	AZQ90YS5-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011370-7	AZQ90YS5-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011370-8	AZQ90YS5-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011370-9	AZQ90YS5-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011370-10	AZQ90YS5-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011370-11	AZQ90YS5-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011370-12	AZQ90YS5-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish Lysozyme/LYZ Antibody PicobandÂ®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Anti-Zebrafish Lysozyme/LYZ Antibody PicobandÂ®

Rate it now..!!