Anti-Zebrafish ILK Antibody Picoband®

Mfr.No.	AZQ6PHD6-2
Background	ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alteatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish ILK Antibody Picoband® catalog #AZQ6PHD6-2. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish ILK recombinant protein (Position: M1-K452)
Molecular Weight	51 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish brain tissue, zebrafish heart tissue, zebrafish muscle tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Integrin-linked protein kinase
Gene Name	ILK
Weight	51 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ6PHD6-2 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). ILK was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-ILK Antibody (AZQ6PHD6-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ILK antigen affinity purified polyclonal antibody (AZQ6PHD6-2) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200)

IHC analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). ILK was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-ILK Antibody (AZQ6PHD6-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of ILK using anti-ILK antibody (AZQ6PHD6-2). ILK was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-ILK Antibody (AZQ6PHD6-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011334-1	AZQ6PHD6-2	Unconjugated	100 ug	$470	Add to Cart
BHA21011334-2	AZQ6PHD6-2-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011334-3	AZQ6PHD6-2-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011334-4	AZQ6PHD6-2-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011334-5	AZQ6PHD6-2-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011334-6	AZQ6PHD6-2-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011334-7	AZQ6PHD6-2-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011334-8	AZQ6PHD6-2-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011334-9	AZQ6PHD6-2-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011334-10	AZQ6PHD6-2-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011334-11	AZQ6PHD6-2-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011334-12	AZQ6PHD6-2-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011334-13	AZQ6PHD6-2-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish ILK Antibody PicobandÂ®

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