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Home / Primary Antibodies / Polyclonal Antibodies

Anti-Zebrafish HSP60/HSPD1 Antibody Picoband®

SKU : BHA21011328

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Mfr.No.	AZQ803B0
Background	HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish HSP60/HSPD1 Antibody Picoband® catalog #AZQ803B0. Tested in WB, IHC, IF applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish HSP60/HSPD1 recombinant protein (Position: K72-Q496)
Molecular Weight	61 kDa
Positive Control	IF: zebrafish embryo tissue WB: zebrafish embryo tissue IHC: zebrafish heart tissue, zebrafish liver tissue, zebrafish spinal cord tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	60 kDa heat shock protein, mitochondrial
Gene Name	HSPD1
Weight	61 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish Immunofluorescence, 5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IF, IHC, WB
Description	AZQ803B0 is guaranteed for IF, IHC, WB Boster Guarantee

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Combination SKU	Supplier No.	Conjugation	Size	Your price	Quantity
BHA21011328-1	AZQ803B0	Unconjugated	100 ug	$470		Add to Cart
BHA21011328-2	AZQ803B0-10ug	Unconjugated	10ug sample (liquid)	$99		Add to Cart
BHA21011328-3	AZQ803B0-Biotin	Biotin conjugated	100 ug	$670		Add to Cart
BHA21011328-4	AZQ803B0-Cy3	Cy3 conjugated	100 ug	$670		Add to Cart
BHA21011328-5	AZQ803B0-Dylight488	Dylight488 conjugated	100 ug	$670		Add to Cart
BHA21011328-6	AZQ803B0-Dylight550	Dylight550 conjugated	100 ug	$670		Add to Cart
BHA21011328-7	AZQ803B0-Dylight594	Dylight594 conjugated	100 ug	$670		Add to Cart
BHA21011328-8	AZQ803B0-FITC	FITC conjugated	100 ug	$670		Add to Cart
BHA21011328-9	AZQ803B0-HRP	HRP conjugated	100 ug	$670		Add to Cart
BHA21011328-10	AZQ803B0-APC	APC conjugated	100 ug	$770		Add to Cart
BHA21011328-11	AZQ803B0-PE	PE conjugated	100 ug	$770		Add to Cart
BHA21011328-12	AZQ803B0-iFluor647	iFluor647 conjugated	100 ug	$770		Add to Cart
BHA21011328-13	AZQ803B0-carrier-free	Carrier Free	100 ug	$470		Add to Cart

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