Anti-Zebrafish HSC70/HSPA8 Antibody Picoband®

Mfr.No.	AZQ90473
Background	HSPA8 (heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene. The HSPA8 gene contains 9 exons and spans 5 kb. The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da. And the HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish HSC70/HSPA8 Antibody Picoband® catalog # AZQ90473. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish HSC70/HSPA8 recombinant protein (Position: K539-K601).
Molecular Weight	71 kDa
Positive Control	WB: zebrafish head tissue, female zebrafish viscera tissue, male zebrafish viscera tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish brain tissue, zebrafish retina tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Heat shock cognate 71 kDa protein
Gene Name	HSPA8
Weight	71 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ90473 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (AZQ90473). HSC70/HSPA8 was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-HSC70/HSPA8 Antibody (AZQ90473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (AZQ90473). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: female zebrafish viscera tissue lysates,Lane 3: male zebrafish viscera tissue lysates,Lane 4: whole female zebrafish tissue lysates,Lane 5: whole male zebrafish tissue lysates,Lane 6: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSC70/HSPA8 antigen affinity purified polyclonal antibody (AZQ90473) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:50

IHC analysis of HSC70/HSPA8 using anti-HSC70/HSPA8 antibody (AZQ90473). HSC70/HSPA8 was detected in a paraffin-embedded section of zebrafish retina tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-HSC70/HSPA8 Antibody (AZQ90473) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011462-1	AZQ90473	Unconjugated	100 ug	$470	Add to Cart
BHA21011462-2	AZQ90473-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011462-3	AZQ90473-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011462-4	AZQ90473-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011462-5	AZQ90473-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011462-6	AZQ90473-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011462-7	AZQ90473-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011462-8	AZQ90473-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011462-9	AZQ90473-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011462-10	AZQ90473-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011462-11	AZQ90473-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011462-12	AZQ90473-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011462-13	AZQ90473-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish HSC70/HSPA8 Antibody PicobandÂ®

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