Anti-Zebrafish HPRT1 Antibody Picoband®

Mfr.No.	AZQ7ZV49-1
Background	Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish HPRT1 Antibody Picoband® catalog #AZQ7ZV49-1. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish HPRT1 recombinant protein (Position: Y17-D185)
Molecular Weight	25 kDa
Positive Control	WB: zebrafish head tissue, zebrafish embryo tissue IHC: zebrafish liver tissue, zebrafish muscle tissue, zebrafish kidney tissue, zebrafish integument tissue, zebrafish brain tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Hypoxanthine-guanine phosphoribosyltransferase
Gene Name	HPRT1
Weight	25 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ7ZV49-1 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +2 HPRT1 was detected in a paraffin-embedded section of zebrafish integument tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-HPRT1 Antibod

Western blot analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPRT1 antigen affinity purified polyclonal antibody (AZQ7ZV49-1) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HPRT1 at approximately 25

IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of HPRT1 using anti-HPRT1 antibody (AZQ7ZV49-1). HPRT1 was detected in a paraffin-embedded section of zebrafish muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-HPRT1 Antibody (AZQ7ZV49-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011345-1	AZQ7ZV49-1	Unconjugated	100 ug	$470	Add to Cart
BHA21011345-2	AZQ7ZV49-1-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011345-3	AZQ7ZV49-1-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011345-4	AZQ7ZV49-1-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011345-5	AZQ7ZV49-1-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011345-6	AZQ7ZV49-1-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011345-7	AZQ7ZV49-1-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011345-8	AZQ7ZV49-1-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011345-9	AZQ7ZV49-1-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011345-10	AZQ7ZV49-1-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011345-11	AZQ7ZV49-1-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011345-12	AZQ7ZV49-1-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011345-13	AZQ7ZV49-1-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish HPRT1 Antibody PicobandÂ®

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