Anti-Zebrafish GLULa/b Antibody Picoband®

Mfr.No.	AZQ7T2P7
Background	The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alteative splicing results in multiple transcript variants.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish-GLULa-b-Antibody Picoband® catalog # AZQ7T2P7. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish GLULa/b recombinant protein (Position: N74-Y371).
Molecular Weight	42 kDa
Positive Control	WB: zebrafish head tissue IHC: zebrafish kidney tissue, zebrafish brain tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Gene Name	GLULa/b
Weight	42 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ7T2P7 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of GLULa/b using anti-GLULa/b antibody (AZQ7T2P7). GLULa/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GLULa/b Antibody (AZQ7T2P7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of GLULa/b using anti-GLULa/b antibody (AZQ7T2P7). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLULa/b antigen affinity purified polyclonal antibody (AZQ7T2P7) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for GLULa/b at approximately 42 kDa. The expected band size for GLUL

IHC analysis of GLULa/b using anti-GLULa/b antibody (AZQ7T2P7). GLULa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GLULa/b Antibody (AZQ7T2P7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011408-1	AZQ7T2P7	Unconjugated	100 ug	$470	Add to Cart
BHA21011408-2	AZQ7T2P7-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011408-3	AZQ7T2P7-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011408-4	AZQ7T2P7-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011408-5	AZQ7T2P7-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011408-6	AZQ7T2P7-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011408-7	AZQ7T2P7-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011408-8	AZQ7T2P7-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011408-9	AZQ7T2P7-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011408-10	AZQ7T2P7-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011408-11	AZQ7T2P7-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011408-12	AZQ7T2P7-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011408-13	AZQ7T2P7-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish GLULa/b Antibody PicobandÂ®

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