Anti-Zebrafish GABARAPa/b Antibody Picoband®

Mfr.No.	AZQ6NZZ7
Background	Gamma-aminobutyric acid receptor-associated protein is a protein that in humans is encoded by the GABARAP gene. Gamma-aminobutyric acid A receptors (GABA(A) receptors) are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish-GABARAPa-b-Antibody Picoband® catalog # AZQ6NZZ7. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	A synthetic peptide corresponding to a sequence at the N-terminus of zebrafish GABARAPa/b.
Molecular Weight	14 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish kidney tissue, zebrafish liver tissue, zebrafish cerebellum tissue, zebrafish brain tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Gene Name	GABARAPa/b
Weight	14 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ6NZZ7 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish cerebellum tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +1 GABARAPa/b was detected in a paraffin-embedded section of zebrafish brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbi

IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). Electrophoresis was performed on a 12% SDS-PAGE SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAPa/b antigen affinity purified polyclonal antibody (AZQ6NZZ7) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substr

IHC analysis of GABARAPa/b using anti-GABARAPa/b antibody (AZQ6NZZ7). GABARAPa/b was detected in a paraffin-embedded section of zebrafish liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-GABARAPa/b Antibody (AZQ6NZZ7) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011422-1	AZQ6NZZ7	Unconjugated	100 ug	$470	Add to Cart
BHA21011422-2	AZQ6NZZ7-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011422-3	AZQ6NZZ7-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011422-4	AZQ6NZZ7-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011422-5	AZQ6NZZ7-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011422-6	AZQ6NZZ7-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011422-7	AZQ6NZZ7-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011422-8	AZQ6NZZ7-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011422-9	AZQ6NZZ7-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011422-10	AZQ6NZZ7-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011422-11	AZQ6NZZ7-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011422-12	AZQ6NZZ7-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011422-13	AZQ6NZZ7-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish GABARAPa/b Antibody PicobandÂ®

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