Anti-Zebrafish E-cadherin/CDH1 Antibody Picoband®

Mfr.No.	AZQ90Z37
Background	CDH1 (Cadherin 1), also known as ECAD or UVO, is a protein that in humans is encoded by the CDH1 gene. Cadherin-1 is a classical member of the cadherin superfamily. By Southe analysis of DNA from a panel of mouse-human somatic cell hybrids, Mansouri et al. (1987, 1988) assigned the UVO gene to 16q (16p11-qter). Frebourg et al. (2006) found that in human embryos CDH1 is highly expressed at 4 and 5 weeks in the frontonasal prominence and at 6 weeks in the lateral and medial nasal prominences, and is therefore expressed during critical stages of lip and palate development. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish E-cadherin/CDH1 Antibody Picoband® catalog #AZQ90Z37. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish E-cadherin/CDH1 recombinant protein (Position: Y23-D864)
Molecular Weight	120 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish inner ear tissue, zebrafish colon tissue, zebrafish esophageal epithelium tissue, zebrafish heart tissue, zebrafish kidney tissue, zebrafish kidney tissue, zebrafish kidney tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Target Details	Cadherin-1
Gene Name	CDH1
Weight	120 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZQ90Z37 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish esophageal epithelium tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen. +4 E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section w

Western blot analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates.Lane 2: whole female zebrafish tissue lysates.Lane 3: whole male zebrafish tissue lysates.Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-E-cadherin/CDH1 antigen affinity purified polyclonal antibody (AZQ90Z37) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish inner ear tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of E-cadherin/CDH1 using anti-E-cadherin/CDH1 antibody (AZQ90Z37). E-cadherin/CDH1 was detected in a paraffin-embedded section of zebrafish colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-E-cadherin/CDH1 Antibody (AZQ90Z37) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011353-1	AZQ90Z37	Unconjugated	100 ug	$470	Add to Cart
BHA21011353-2	AZQ90Z37-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011353-3	AZQ90Z37-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011353-4	AZQ90Z37-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011353-5	AZQ90Z37-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011353-6	AZQ90Z37-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011353-7	AZQ90Z37-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011353-8	AZQ90Z37-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011353-9	AZQ90Z37-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011353-10	AZQ90Z37-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011353-11	AZQ90Z37-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011353-12	AZQ90Z37-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish E-cadherin/CDH1 Antibody PicobandÂ®

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