Anti-Zebrafish CKMa/b Antibody Picoband®

Mfr.No.	AZA2BHA3
Background	Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish-CKMa-b-Antibody Picoband® catalog # AZA2BHA3. Tested in WB, IHC applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish CKMa/b recombinant protein (Position: M1-K196).
Molecular Weight	43 kDa
Positive Control	WB: zebrafish head tissue, whole female zebrafish tissue, whole male zebrafish tissue, zebrafish embryo tissue IHC: zebrafish spleen tissue, zebrafish kidney tissue, zebrafish pancreas tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Gene Name	CKMa/b
Weight	43 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish Immunohistochemistry, 2-5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	IHC, WB
Description	AZA2BHA3 is guaranteed for IHC, WB Boster Guarantee

IHC analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). CKMa/b was detected in a paraffin-embedded section of zebrafish pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-CKMa/b Antibody (AZA2BHA3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). CKMa/b was detected in a paraffin-embedded section of zebrafish spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-CKMa/b Antibody (AZA2BHA3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Western blot analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates,Lane 2: whole female zebrafish tissue lysates,Lane 3: whole male zebrafish tissue lysates,Lane 4: zebrafish embryo tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CKMa/b antigen affinity purified polyclonal antibody (AZA2BHA3) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196

IHC analysis of CKMa/b using anti-CKMa/b antibody (AZA2BHA3). CKMa/b was detected in a paraffin-embedded section of zebrafish kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ?g/ml rabbit anti-CKMa/b Antibody (AZA2BHA3) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011441-1	AZA2BHA3	Unconjugated	100 ug	$470	Add to Cart
BHA21011441-2	AZA2BHA3-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011441-3	AZA2BHA3-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011441-4	AZA2BHA3-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011441-5	AZA2BHA3-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011441-6	AZA2BHA3-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011441-7	AZA2BHA3-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011441-8	AZA2BHA3-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011441-9	AZA2BHA3-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011441-10	AZA2BHA3-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011441-11	AZA2BHA3-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011441-12	AZA2BHA3-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011441-13	AZA2BHA3-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish CKMa/b Antibody PicobandÂ®

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