Anti-Zebrafish AKT1/2/3 Antibody Picoband®

Mfr.No.	AZQ802Y3
Background	RAC-alpha serine/threonine-protein kinase is an enzyme that in humans is encoded by the AKT1 gene. This gene encodes one of the three members of the human AKT serine-threonine protein kinase family which are often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene. Phosphorylation of additional residues also occurs, for example, in response to insulin growth factor-1 and epidermal growth factor. Protein phosphatases act as negative regulators of AKT proteins by dephosphorylating AKT or PIP3. The PI3K/AKT signalling pathway is crucial for tumor cell survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. AKT proteins also participate in the mammalian target of rapamycin (mTOR) signalling pathway which controls the assembly of the eukaryotic translation initiation factor 4F (eIF4E) complex and this pathway, in addition to responding to extracellular signals from growth factors and cytokines, is disregulated in many cancers. Mutations in this gene are associated with multiple types of cancer and excessive tissue growth including Proteus syndrome and Cowden syndrome 6, and breast, colorectal, and ovarian cancers. Multiple alteatively spliced transcript variants have been found for this gene.
Antibody Validation	Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Clonality	Polyclonal
Description	Boster Bio Anti-Zebrafish AKT1/2/3 Antibody Picoband® catalog # AZQ802Y3. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Weste blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Host	Rabbit
Immunogen Region	E.coli-derived zebrafish AKT1/2/3 recombinant protein (Position: E17-A474).
Molecular Weight	56 kDa
Positive Control	WB: zebrafish head tissue
Reactivity	Zebrafish
Reconstitution	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Note	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Gene Name	AKT1/AKT2/AKT3
Weight	56 kDa

Dilutions	Weste blot, 0.25-0.5 ?g/ml, Zebrafish
Form	Lyophilized
Storage	At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Applications	WB
Description	AZQ802Y3 is guaranteed for WB Boster Guarantee

Western blot analysis of AKT1/2/3 using anti-AKT1/2/3 antibody (AZQ802Y3). Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: zebrafish head tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT1/2/3 antigen affinity purified polyclonal antibody (AZQ802Y3) at 0.5 ?g/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AKT1/2/3 at approximately 62 kDa. The expected band size for

Have you used this product?

Rate it now..!!

Combination SKU	Supplier No.	Conjugation	Size	Your price
BHA21011481-1	AZQ802Y3	Unconjugated	100 ug	$470	Add to Cart
BHA21011481-2	AZQ802Y3-10ug	Unconjugated	10ug sample (liquid)	$99	Add to Cart
BHA21011481-3	AZQ802Y3-Biotin	Biotin conjugated	100 ug	$670	Add to Cart
BHA21011481-4	AZQ802Y3-Cy3	Cy3 conjugated	100 ug	$670	Add to Cart
BHA21011481-5	AZQ802Y3-Dylight488	Dylight488 conjugated	100 ug	$670	Add to Cart
BHA21011481-6	AZQ802Y3-Dylight550	Dylight550 conjugated	100 ug	$670	Add to Cart
BHA21011481-7	AZQ802Y3-Dylight594	Dylight594 conjugated	100 ug	$670	Add to Cart
BHA21011481-8	AZQ802Y3-FITC	FITC conjugated	100 ug	$670	Add to Cart
BHA21011481-9	AZQ802Y3-HRP	HRP conjugated	100 ug	$670	Add to Cart
BHA21011481-10	AZQ802Y3-APC	APC conjugated	100 ug	$770	Add to Cart
BHA21011481-11	AZQ802Y3-PE	PE conjugated	100 ug	$770	Add to Cart
BHA21011481-12	AZQ802Y3-iFluor647	iFluor647 conjugated	100 ug	$770	Add to Cart
BHA21011481-13	AZQ802Y3-carrier-free	Carrier Free	100 ug	$470	Add to Cart

Anti-Zebrafish AKT1/2/3 Antibody PicobandÂ®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Anti-Zebrafish AKT1/2/3 Antibody PicobandÂ®

Rate it now..!!