Virus-like Particles (VLP’s) expressing recombinant Ebola virus (EBOV) glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40). Are produced in Sf9 insect cells through infection with a recombinant baculovirus. Relevance: These VLP’s mimic Ebola virus but do not contain genetic material, thus are not infectious and make an ideal candidate as a vaccine and tool to enhance filovirus research.
Alternative Names
EBOV VLP
Expression
SF9 cells
Formulation
Supplied in PBS (supplemented with arginine and glutamic acid).
Target Details
Ebola virus-like particles
Pathogen Strain
Zaire
Target Pathogen
Ebola Virus
Virus Type
Filoviruses
Purification
Column chromatography
Storage
Store at 2-3 weeks at -20°C, -80°C long term. Subaliquoting is recommended to avoid repeated freeze/thaw cycles.
Notes
After thawing, VLP’s tend to sediment to bottom of the tube and appear as aggregate pellet. Resuspend by gentle vortexing or pipetting up and down.
Reviews of Ebola virus-like particles (EBOV VLP) (Zaire)