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Home / VECTOR &VIRUS / Lentivirus

SOX2-OCT4 Reporter Lentivirus

SKU : BHV19400083

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Mfr.No.	LTV-0090, LTV-0090-1S, LTV-0090-2S, LTV-0090-3S, LTV-0090-4S, LTV-0090-5S, LTV-0090-6S, LTV-0090-15S, LTV-0090-16S
Description	SOX2-OCT4 Composite Reporter Lentivirus: High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse SRY-box transcription factor 2 (SOX2) transcriptional activity in mammalian cells. A stem cell enhancer minigene was designed, based on the observation that the proximal NANOG promoter region has highly conserved composite binding element for SOX2 and OCT4 that is essential for properly regulated expression of NANOG in ESCs (PMID: 25497455). A minigene containing six tandem repeats of this composite element with eight bases of native flanking sequence on either side of the element were used to make this product. It has been validated to read out transcriptional activity of SOX2 in HEK293FT cells. The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for use in difficult-to-transfect cells including primary and/or thawed cells.
Serotype	VSV-G
Reporter	Firefly Luc, RFP, BFP2, GFP
Response Element	SOX2 and OCT4 composite DNA binding element
Selection	Blasticidin, Puromycin
Signaling pathway	SOX2 and OCT4
Species	Human/mouse
Applications Range	Monitor transcriptional activity of SOX2-OCT4 in mammalian cells, screen for activators or inhibitors of SOX2-OCT4 transcriptional activity. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Your price	Quantity
BHV19400083-1	LTV-0090-1S	5x10^6	Puromycin	GFP	$595		Add to Cart
BHV19400083-2	LTV-0090-2S	5x10^6	Blasticidin	RFP	$595		Add to Cart
BHV19400083-3	LTV-0090-3S	2x10^6	Puromycin	Firefly Luc	$595		Add to Cart
BHV19400083-4	LTV-0090-4S	2x10^6	Blasticidin	Firefly Luc	$595		Add to Cart
BHV19400083-5	LTV-0090-5S	5x10^6	Blasticidin	GFP	$595		Add to Cart
BHV19400083-6	LTV-0090-6S	5x10^6	Puromycin	RFP	$595		Add to Cart
BHV19400083-7	LTV-0090-15S	5x10^6	Puromycin	BFP2	$595		Add to Cart
BHV19400083-8	LTV-0090-16S	5x10^6	Blasticidin	BFP2	$595		Add to Cart

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