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Home / VECTOR &VIRUS / AAV Plasmids

pAAVone-AAV2-CMV-mCherry

SKU : BHV17900042

pAAVone plasmids are used to produce AAVs using AAVnerGene s novel AAVone signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAV13, AAVrh.74, and AAV-PHP.eB. If you require any other AAV serotypes or variants, please contact us, and we can produce them within 2 weeks.

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Mfr.No.	SE001006-AAV2
Description	pAAVone-AAV2-CMV-mCherry is a plasmid used to produce AAV2-CMV-mCherry using AAVnerGene s novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV2 Cap), and a single-strand AAV-CMV-mCherry genome. AAV2-CMV-mCherry viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV2 is one of the most well-studied and widely used serotypes of adeno-associated virus. It has been a workhorse for gene therapy research due to its well-characterized biology and its ability to transduce a broad range of tissues, including the central nervous system (CNS), liver, and retina. AAV2 was the first AAV serotype to be cloned and is often used as a reference or baseline for comparing other AAV serotypes.
Promoter	CMV
Insert Gene	mCherry
Serotype	AAV2
Bacterial Resistance	Kana
Genome Size	2167 bp
Genome Type	ssAAV
ITRs	L-ITR (119 bp) + R-ITR (130 bp)
Plasmid Size	10641 bp
Production Systems	pAAVone
Plasmid Backbone	pAAVone
AAV Genome	AAV-CMV-SV40Intron-mCherry-WPRE3-SV40PolyA
Storage	-20°C for long term (1 year)
Shipping	Dry ice
Notes	Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone. AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin). AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.

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SKU	Supplier No.	Size	Your price	Quantity
BHV17900042	SE001006-AAV2	10 ug	$999		Add to Cart
BHV17900042	SE001006-AAV2	100 ug	$1199		Add to Cart
BHV17900042	SE001006-AAV2	1 mg	$1399		Add to Cart
BHV17900042	SE001006-AAV2	10 mg	$1899		Add to Cart

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