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Home / VECTOR &VIRUS / AAV Plasmids

pAAVone-AAV1-CMV-EGFP

SKU : BHV17900051

pAAVone plasmids are used to produce AAVs using AAVnerGene s novel AAVone signle-plasmid AAV production system. They integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and Cap), and AAV genome. To produce AAVs, users only need select the pAAVone plasmid carrying the corresponding capsid, clone your transgenes into it and transfect them into host cells. Pre-made pAAVone plasmids are available in the following serotypes: AAV1, AAV2, AAV2-Retro, AAV3B, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAV13, AAV-DJ, AAV13, AAVrh.74, and AAV-PHP.eB. If you require any other AAV serotypes or variants, please contact us, and we can produce them within 2 weeks.

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Mfr.No.	SE001001-AAV1-10ug
Description	pAAVone-AAV1-CMV-EGFP is a plasmid used to produce AAV1-CMV-EGFP using AAVnerGene s novel AAVone production system. It integrates all the AAV production necessary components, including Ad helper genes (E2A, E4orf6 and VA RNA), AAV helper genes (Rep and AAV1), and a single-strand AAV-CMV-EGFP genome. AAV1-CMV-EGFP viruses can be simply and efficiently produced by transfection of this single-into host cells, such us HEK 293 and HEK 293T. AAV1 is a serotype of adeno-associated virus (AAV) that was originally isolated from rhesus monkeys. AAV1 can efficiently target the CNS, liver, lungs, heart, and muscle tissues, among others. Its ability to cross the blood-brain barrier(BBB) makes it particularly valuable for delivering therapeutic genes to the brain.
Promoter	CMV
Insert Gene	EGFP
Serotype	AAV1
Bacterial Resistance	Kana
Genome Size	2167 bp
Genome Type	ssAAV
ITRs	L-ITR (119 bp) + R-ITR (130 bp)
Plasmid Size	10641 bp
Production Systems	pAAVone
Plasmid Backbone	pAAVone
AAV Genome	AAV-CMV-SV40Intron-EGFP-WPRE3-SV40PolyA
Storage	-20°C for long term (1 year)
Shipping	Dry ice
Notes	Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone. AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin). AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.

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SKU	Supplier No.	Size	Your price	Quantity
BHV17900051	SE001001-AAV1-10ug	10 ug	$999		Add to Cart
BHV17900051	SE001001-AAV1-10ug	100 ug	$1199		Add to Cart
BHV17900051	SE001001-AAV1-10ug	1 mg	$1399		Add to Cart
BHV17900051	SE001001-AAV1-10ug	10 mg	$1899		Add to Cart

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