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Home / VECTOR &VIRUS / AAV Plasmids

pAAVdual-sc-CMV-EGFP

SKU : BHV17900062

pAAVdual-sc plasmids are used to produce self-complementary AAV (scAAV) viruses using AAVnerGene s novel AAVdual System. In this series plasmids, the scAAV genomes are cloned into our novel Ad helper plasmid, mini-pHelper. scAAV viruses can be generated by co-transfection of pAAVdual-sc plasmids with the regular AAV helper plasmids.

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Mfr.No.	SL023001-XS
Description	pAAVdual-sc-CMV-EGFP is used to produce scAAV-CMV-EGFP virus using AAVnerGene s novel AAVdual production system. In this plasmid, the self-complementary AAV genome with a CMV promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. scAAV-CMV-EGFP virus can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions. The CMV promoter, also known as the cytomegalovirus promoter, is a widely used promoter sequence in molecular biology and biotechnology. It is derived from the immediate early promoter of the cytomegalovirus and is commonly used to drive the expression of genes in a variety of experimental and practical applications.
Promoter	CMV
Insert Gene	EGFP
AAV Cassette	scAAV-CMV-EGFP
Bacterial Resistance	Kana
Genome Size	2165 bp
Genome Type	scAAV
ITRs	mITR (72 bp) + R-ITR (130 bp)
Plasmid Size	10678 bp
Production Systems	pAAVdual
Plasmid Backbone	pAAVdual-sc
Storage	-20°C for long term (1 year)
Shipping	Dry ice
Notes	Cloning Services: AAVnerGene provides cloning services based on this plasmid backbone with $300/clone. AAV Package Services: AAVnerGene provides affordable AAV production services at various scales(from 1e12 to 1e16 vg), in both suspension and adherent cultured cells (HEK 293T or HEK 293), with flexible purification methods (CsCl, Iodixanol, or AAV-X resin). AAV Analytical Services: We also offer advanced AAV characterization services, including AAV purity, vector genome identity, ITR integrity, DNA impurities, empty/full ratios, and a variety of comprehensive analyses based on custom requests.

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SKU	Supplier No.	Size	Your price	Quantity
BHV17900062	SL023001-XS	10 ug	$999		Add to Cart
BHV17900062	SL023001-XS	100 ug	$1199		Add to Cart
BHV17900062	SL023001-XS	1 mg	$1399		Add to Cart
BHV17900062	SL023001-XS	10 mg	$1899		Add to Cart

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