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Home / VECTOR &VIRUS / Lentivirus

E2F Reporter Lentivirus

SKU : BHV19400022

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Mfr.No.	LTV-0026, LTV-0026-1S, LTV-0026-2S, LTV-0026-3S, LTV-0026-4S, LTV-0026-5S, LTV-0026-6S, LTV-0026-4SIC
Description	Fluorescent E2F Reporter Lentivirus: High-quality, fluorescent lentiviral transcription factor (TF) reporter system that provides sensitive fluorescent readout for human/mouse E2F transcription factors (E2F) activity in transduced cells. The E2F reporter is constructed with tandem repeats of consensus E2F DNA-binding elements to read out the activities of E2F transcription factors in mammalian cells. As a member of the E2F family of transcription factors, E2F2 often act as transcriptional activator to activate certain target genes such as cyclin A and E in the cell cycle entry for proliferation. Tumor suppressor Retinoblastoma protein (Rb) in unphosphorylated form binds to E2F2 in G0/G1, forming a complex that actively represses E2F2-responsive genes. CDKs phosphorylate Rb proteins causing the release and accumulation of sequestered E2F2. E2F2 overexpression promotes the development and progression of tumors such as breast, lung cancer, and liver cancer. The E2F reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying E2F transcription factor activity in difficult-to-transfect cells including primary and/or thawed cells.
Promoter	EF1a
Serotype	VSV-G
Reporter	Firefly Luc, RFP, Renilla Luc, GFP
Response Element	E2F consensus DNA-binding elements
Selection	Blasticidin, Puromycin
Signaling pathway	E2F transcription factors
Species	Human/mouse
Applications Range	Monitor E2F transcription factor activity in mammalian cells, screen for activators or inhibitors of E2F pathway. The fluorescent reporter enables convenient readout using flow cytometry, fluorescence microscopy, etc.
Virus Type	TF Reporter Lentivirus

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Combination SKU	Supplier No.	Amount (TU)	Selection	Reporter	Promoter	Your price	Quantity
BHV19400022-1	LTV-0026-1S	5x10^6	Puromycin	GFP	EF1a	$595		Add to Cart
BHV19400022-2	LTV-0026-2S	5x10^6	Blasticidin	RFP	EF1a	$595		Add to Cart
BHV19400022-3	LTV-0026-3S	2x10^6	Puromycin	Firefly Luc	EF1a	$595		Add to Cart
BHV19400022-4	LTV-0026-4S	2x10^6	Blasticidin	Firefly Luc	EF1a	$595		Add to Cart
BHV19400022-5	LTV-0026-5S	5x10^6	Blasticidin	GFP	EF1a	$595		Add to Cart
BHV19400022-6	LTV-0026-6S	5x10^6	Puromycin	RFP	EF1a	$595		Add to Cart
BHV19400022-7	LTV-0026-4SIC	2x10^6	Blasticidin	Renilla Luc	EF1a	$595		Add to Cart

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