Human Tumor Necrosis Factor Receptor 1 (TNFR1) (Sandwich ELISA) ELISA Kit

Mfr.No.

MEB499Hu

Description

This assay has high sensitivity and excellent specificity for detection of Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1).
No significant cross-reactivity or interference between Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) and analogues was observed.

Alternative Names

CD120A; P55; TNFRSF1A; TBP1; FPF; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR55; TNFR60; P55-R; P60; Tumor necrosis factor receptor 1; Tumor necrosis factor-binding protein 1

Reactivity

Human

Detection Method

Colormetric

Detection Range

15.6-1,000pg/mL

Sample Type

Cell lysates, Other biological fluids, Tissue homogenates, Cell culture supernates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×60µL	Assay Diluent A	1×6mL
Detection Reagent B	1×60µL	Assay Diluent B	1×6mL
TMB Substrate	1×4.5mL	Stop Solution	1×3mL
Wash Buffer (30 × concentrate)	1×10mL	Instruction manual	1

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	93-104	99
EDTA plasma(n=5)	95-103	99
heparin plasma(n=5)	86-101	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	83-104%	99-105%	89-97%	80-104%
EDTA plasma(n=5)	93-101%	91-104%	85-92%	79-96%
heparin plasma(n=5)	91-102%	93-105%	78-105%	81-97%

Features

Detects protein (micro-volume samples 15-25 ?l)

Species

Human

Sensitivity

5.6pg/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene ID	7132
UniProt ID	P19438
Antigen	TNFR1
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C; 3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 20µL Stop Solution. Read at 450nm immediately.

Applications	ELISA
Research Area	Signal transduction
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Tumor Necrosis Factor Receptor 1 (TNFR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: C<10% Inter-Assay: C<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10109700	MEB499Hu	96T*1	$535	Add to Cart
BHE10109700	MEB499Hu	96T*5	$2407.50	Add to Cart
BHE10109700	MEB499Hu	96T*10	$4547.50	Add to Cart

Mini Samples ELISA Kit for Tumor Necrosis Factor Receptor 1 (TNFR1) (Human)

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mini Samples ELISA Kit for Tumor Necrosis Factor Receptor 1 (TNFR1) (Human)

Rate it now..!!