Human/Mouse/Rat Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) (Sandwich ELISA) ELISA Kit

Mfr.No.

SEJ809Mi

Sensitivity

12.2pg/mL

Detection Range

31.2-2,000pg/mL

Reactivity

Human, Mouse, Rat

Assay Duration

Assay Type

Sandwich

Description

This assay has high sensitivity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz).
100% cross-reactivity of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) was observed among Human, Mouse, Rat.

Alternative Names

KCIP-1; 14-3-3-zeta; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1

Detection Method

Colormetric

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Features

Detects protein (regular version)

Species

Human///Mouse///Rat

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: C<10%
Inter-Assay: C<12%

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Usage Notes

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Protocol

Datasheet

Gene ID	7534
UniProt ID	P63104

Applications	ELISA
Research Area	Enzyme///Kinase

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Differential expression of serum proteins in rats subchronically exposed to arsenic identified by iTRAQ-based proteomic technology—14-3-3 ζ protein to serve as a potential biomarker Differential expression of serum proteins in rats subchronically exposed to arsenic identified by iTRAQ-based proteomic technology—14-3-3 ζ protein to serve …

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SKU	Supplier No.	Size	Your price
BHE10107971	SEJ809Mi	96T*1	$720	Add to Cart
BHE10107971	SEJ809Mi	96T*5	$3240	Add to Cart
BHE10107971	SEJ809Mi	96T*10	$6120	Add to Cart

ELISA Kit for Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) (Human/Mouse/Rat)

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