Rat Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) (Sandwich ELISA) ELISA Kit

Mfr.No.

SEH337Ra

Description

This assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a).
No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) and analogues was observed.

Alternative Names

LEM6; PGC-1(alpha); PGC-1v; PGC1; PGC1A; PPARGC1; Ligand effect modulator 6

Reactivity

Rat

Detection Method

Colormetric

Detection Range

0.312-20ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

Kit Components

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Features

Detects protein (regular version)

Species

Rat

Sensitivity

0.118ng/mL

Precautions

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Gene ID	83516
UniProt ID	Q9QYK2
Antigen	PPARgC1a
Usage Notes	1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.

Applications	ELISA
Research Area	CD///Adhesion molecule
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%

Storage	4°C for 1 month/ -20°C for long-term(within shelf life)
Shelf Life	one year

Reference	Skeletal muscle mitochondrial dysfunction precedes right ventricular impairment in experimental pulmonary hypertension Inorganic nitrate promotes the browning of white adipose tissue through the nitrate-nitrite-nitric oxide pathway Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats

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SKU	Supplier No.	Size	Your price
BHE10107545	SEH337Ra	96T*1	$760	Add to Cart
BHE10107545	SEH337Ra	96T*5	$3420	Add to Cart
BHE10107545	SEH337Ra	96T*10	$6460	Add to Cart

ELISA Kit for Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) (Rat)

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