JavaScript is currently not supported/disabled by this browser. Please enable JavaScript for full functionality.

Call Us 1 - 866 - 986 - 9598
[email protected]

Sign Up

Login
- My Order
- Return Requests
- Track my Order

Home / Cultures / Transfection Reagents

Polybrene (hexadimethrine bromide)

SKU : BHM20800019

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Request More Infos

Yeasen

view supplier details

Order Now

Credit card payments now incur a 3% fee.

Contact us to order

Tel

+1 866.986.9598

Email

[email protected]

Get a Quote

Ask Merchant

Request More Infos

Sample Request

Mfr.No.	40804ES86, 40804ES76
Description	Polybrene (hexadimethrine bromide), also known as Polybrene, is a cationic polymer commonly used in DNA transfection experiments with mammalian cells to enhance the transfection efficiency of liposomes. It is widely employed in retrovirus-mediated gene transfection and lentivirus-mediated gene transfection. The mechanism of action may involve neutralizing the sialic acid on the cell surface, facilitating adsorption by overcoming electrostatic repulsion with virus particles. Polybrene is also recognized as an anticoagulant (heparin antagonist) and is frequently used in the production of non-specifically aggregated red blood cells. Additionally, in protein sequencing, low doses of Polybrene have been shown to significantly improve the degradation of peptides in automated sequencing analysis. The addition of Polybrene to PVDF membranes can also enhance membrane affinity.
General Information	The product is provided in solution form, and the powder is reconstituted in 0.9% NaCl to make a 10 mg/mL solution, which is then sterilized using a 0.22 μm filter. It is typically diluted at a ratio of 1:1000-1:2000 for use, with specific dilution ratios depending on the cell type, as outlined in relevant literature.
Precautions	Wear laboratory attire and disposable gloves for safety and health. This product is for research purposes only.
Product Use	Operational steps (for reference, specific concentrations may vary, refer to relevant literature): Note: Polybrene may exhibit significant toxicity to certain cells (such as terminally differentiated neurons, DC cells), and a toxicity test is recommended for initial use. Experiment 1: Retroviral Infection Preparation of recombinant retrovirus stock: Culture cells containing a monolayer of transfection retrovirus packaging cells in a 100 mm dish with 5 mL growth medium (5% serum). After 24 hours, remove the culture medium and filter it through a 0.45 μm filter. Culturing cells for infection: In a 100 mm dish, add 10 mL complete growth medium with a cell density of 5×105 per dish. Virus infection: After 24 hours of cell culture, remove the complete culture medium. Infect cells with 2 mL virus supeatant containing Polybrene (final concentration: 5-10 μg/mL) at 37°C for 3-6 hours. Collect virus particles: Add 8 mL complete growth medium. After 2-3 days of culture, collect the culture medium to obtain virus particles. Experiment 2: Transfection Culture cells in complete growth medium with a cell density of approximately 50%. After 18-24 hours of cell culture, prepare a DNA-growth medium-Polybrene mixture. Add complete growth medium (2 mL for a 60 mm dish, 3 mL for a 100 mm dish) and preheat to 37°C. Gently mix 10 ng~10 µg plasmid. Add Polybrene to a final concentration of 5-10 μg/mL. Gently mix. Each component should be added in the specified order. Remove the culture medium and add the DNA-growth medium-Polybrene solution to the cells at 37°C for 6-20 hours. Gently mix every 1.5 hours within the first 6 hours of cell culture. Remove the DNA-growth medium-Polybrene solution. Cover cells with DMSO shock solution (15% DMSO in 1× HBSS). Gently shake the culture dish for 10 seconds each time the solution is added to ensure even distribution. Incubate cells at 37°C for 4 minutes. Immediately remove the DMSO shock solution and gently wash the cells twice with complete growth medium. For a 60 mm dish, wash with 5 mL of culture medium each time, and for a 100 mm dish, wash with 10 mL of culture medium each time. Add complete growth medium to the cells. Protein expression: After 24-72 hours of culture, collect cells for protein expression analysis as needed. Cell selection: After 24-72 hours of culture, based on the cell status, switch to fresh selection medium and continue with cell selection.

Shipping Condition	Transportation and storage are recommended at -20 ºC, with a shelf life of 2 years. It is advised to aliquot and store to avoid repeated freeze-thaw cycles.
Storage	Recommended at -20 ºC, with a shelf life of 2 years
Product Use	Operational steps (for reference, specific concentrations may vary, refer to relevant literature): Note: Polybrene may exhibit significant toxicity to certain cells (such as terminally differentiated neurons, DC cells), and a toxicity test is recommended for initial use. Experiment 1: Retroviral Infection Preparation of recombinant retrovirus stock: Culture cells containing a monolayer of transfection retrovirus packaging cells in a 100 mm dish with 5 mL growth medium (5% serum). After 24 hours, remove the culture medium and filter it through a 0.45 μm filter. Culturing cells for infection: In a 100 mm dish, add 10 mL complete growth medium with a cell density of 5×105 per dish. Virus infection: After 24 hours of cell culture, remove the complete culture medium. Infect cells with 2 mL virus supeatant containing Polybrene (final concentration: 5-10 μg/mL) at 37°C for 3-6 hours. Collect virus particles: Add 8 mL complete growth medium. After 2-3 days of culture, collect the culture medium to obtain virus particles. Experiment 2: Transfection Culture cells in complete growth medium with a cell density of approximately 50%. After 18-24 hours of cell culture, prepare a DNA-growth medium-Polybrene mixture. Add complete growth medium (2 mL for a 60 mm dish, 3 mL for a 100 mm dish) and preheat to 37°C. Gently mix 10 ng~10 µg plasmid. Add Polybrene to a final concentration of 5-10 μg/mL. Gently mix. Each component should be added in the specified order. Remove the culture medium and add the DNA-growth medium-Polybrene solution to the cells at 37°C for 6-20 hours. Gently mix every 1.5 hours within the first 6 hours of cell culture. Remove the DNA-growth medium-Polybrene solution. Cover cells with DMSO shock solution (15% DMSO in 1× HBSS). Gently shake the culture dish for 10 seconds each time the solution is added to ensure even distribution. Incubate cells at 37°C for 4 minutes. Immediately remove the DMSO shock solution and gently wash the cells twice with complete growth medium. For a 60 mm dish, wash with 5 mL of culture medium each time, and for a 100 mm dish, wash with 10 mL of culture medium each time. Add complete growth medium to the cells. Protein expression: After 24-72 hours of culture, collect cells for protein expression analysis as needed. Cell selection: After 24-72 hours of culture, based on the cell status, switch to fresh selection medium and continue with cell selection.

Have you used this product?

Rate it now..!!

Login to Review

Combination SKU	Supplier No.	Size	Your price	Quantity
BHM20800019-2	40804ES76	500 uL	$100		Add to Cart
BHM20800019-1	40804ES86	5x 500 uL	$480		Add to Cart

ebiohippo.com provides biomedical researchers with a one-stop informatic shopping experience by transforming biotechnology products’ data into a ready-to-analyze state. We collect and unify suppliers’ data on a single platform to reduce the time of searching, uncertainty, and cost of scientific experiments.

© 2025 Biohippo. All rights reserved.