CAG-LSL-dCas9-SPH-Tg

Mfr.No.	NM-TG-00025
Description	Model Description: Cre-dependent SunTag-p65-HSF1 (SPH) transgenic mice were generated with piggyBac transposon system in F1 zygotes and crossed with wild type C57BL/6 mice., SPH transgenic mouse containing HA-tagged dCas9 fused with 10xGCN4, which is linked with p65-HSF1 and EGFP in tandem via P2A and T2A respectively. The transgene is driven by the ubiquitous CAG promoter and interrupted by a loxP-stop-loxP (LSL) cassette to render Cas9 expression inducible by the Cre recombinase. Expression of dCas9 can be detected using primary antibody: rabbit monoclonal antibody to HA-tag (1:1000, #3724, CST45) and secondary antibody: goat anti-rabbit Alexa Fluor 488 (1:1000, #A-11034, Thermo Fisher). Official Symbol: dCas9 Pubmed link: dCas9
Nomenclature	C57BL/6JSmoc-Tg(CAG-LSL-dCas9-SPH)Smoc
Strain	Repository Live
KI/KO Gene	dCas9
Synonyms	Null

Applications Range	gene regulation tool,CRISPRa
Research Application	CRISPR/Cas knockins

Have you used this product?

SKU	Supplier No.	Your price	Quantity
BHAM00490	NM-TG-00025	-		Add to Quote