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Home / Primary Antibodies / IHC Antibodies

TNF Antibody

SKU : BHA10557117

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA023955KA01HU
Alternative Names	TNF ; TNFSF2; DIF; Tumor necrosis factor; TNF a; TNF alpha
Categories	Primary Antibodies
Description	TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death. TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease.
Hgnc	11935
Host	Rabbit
Immunogen	C term -peptide of Human SFPQ
Intended Use	For research use only. Not for human, diagnostic or therapeutic use.
Involvement In Disease	Psoriatic arthritis (PSORAS)
Isotype	IgG
Mgi	88337
Raised In	Rabbit
Reactivity	Human, Mouse, Rat
Regulatory	RUO
Relevance	TNF-α, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein. Membrane-bound TNF-α is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer. Both membrane and soluble forms of TNF-α are biologically active. TNF-α is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages. Cellular response to TNF-α is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-κB) promotes the survival of cells, while TNF-α-mediated activation of caspase-8 leads to programmed cell death. TNF-α plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis. The role of TNF-α in autoimmunity is underscored by blocking TNF-α action to treat rheumatoid arthritis and Crohn’s disease.
Size	100μl(100μg)
Species	Homo Sapiens (Human)
Subcellular Location	Cell membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, membrane form: Membrane, Single-pass type II membrane protein, SUBCELLULAR LOCATION: Tumor necrosis factor, soluble form: Secreted, SUBCELLULAR LOCATION: C-domain 1: Secreted, SUBCELLULAR LOCATION: C-domain 2: Secreted

Rgd	708418
Target Details	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Uniprot	P01375
Database Links	HGNC:11892 OMIM:191160 KEGG:hsa:7124 STRING:9606.ENSP00000398698 UniGene:Hs.241570
Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective
Pathway	MAPK signaling pathway mTOR signaling pathway TGF-beta signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis Necroptosis Antigen processing and presentation Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Toll-like receptor signaling pathway Adipocytokine signaling pathway AGE-RAGE signaling pathway in diabetic complications Inflammatory Pain
Protein Families	Tumor necrosis factor family
Research Area	Immunology

Buffer	Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Format	liquid
Purification	Affinity purification
Purity	Affinity purification
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage Buffer	Store at -20oC or -80oC. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Applications

ELISA, WB, IHC

Description

ELISA ; WB ; IHC, WB ; ELISA ; IHC

Dilution

WB	1:1000-1:2000
IHC	1:50-1:100

Tested Application

ELISA,WB,IHC;WB:1:1000-1:2000,IHC:1:50-1:100

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10557117	CSB-PA023955KA01HU	100ul	$398		Add to Cart

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