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Home / Assay Kits / Biochemical Kits

Spin-columns for Liposome Purification

SKU : BHE16300404

This product includes 20 pre-packed spin columns. The columns are pre-packed in water and specifically designed for removal of free drugs in a liposome sample.

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Mfr.No.	SLP20
Description	The Spin-columns for Liposome Purification (Catalog number SLP20) is designed for quick removal of free or non-encapsulated drugs. It removes > 99 % of the non-encapsulated drugs in a few minutes. Removal of the non-encapsulated drugs is based on a combination effect of absorbance and size exclusion. When a purified 2 mg/ml liposomal doxorubicin is loaded, 99.3% liposomal doxorubicin is recovered. When a 2 mg/ml free doxorubicin is loaded, 0.2 % free doxorubicin is recovered. The Spin-columns for Liposome Purification (Catalog number SLP20) includes 20 pre-packed spin columns. The pre-packed columns are specifically designed for removal of free drugs in a liposome sample. A minimal amount of liposomes may also bind to the column. For liposomal drug encapsulation and liposomal drug dissolution tests, please use assay kits specifically designed for drug encapsulation assays (Catalog number LDE10) or drug dissolution assays (Catalog number LDD05).
Liposomes	Liposomal research tools

Product Manual	Datasheet
Protocols Description	PROTOCOL 1. Column preparation (1) Remove the caps of 1.5-ml Eppendorf tubes and use them as receiver tubes. Remove the bottom tip and the cap of each spin column and insert the column into a receiving tube. (2) Spin the columns at 1000 rpm using a benchtop Eppendorf centrifuge for 1 min and discard the elute. (3) Repeat Step (2). (4) Add 150 µl of the same buffer of the liposome preparation, spin the columns at 1000 rpm for 2 min and discard the elute. (5) Spin the columns at 1000 rpm for 4 min and change to a clean receiving tube. 2. Separation of encapsulated drugs (1) Load 100 µl of the liposome sample onto each column. (2) Spin the column at 1000 rpm for 4 min. Collect the elute. Discard the column.

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SKU	Supplier No.	Size	Your price	Quantity
BHE16300404	SLP20	20 columns	$241.67		Add to Cart

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