Polysynaptic Retrograde Tracing with Pseudorabies Virus at Braincase
Unraveling Neural Circuits with Precision
Braincase specializes in advanced polysynaptic retrograde tracing techniques using Pseudorabies Virus (PRV) to map complex neural circuits. Our tailored PRV-based tools enable researchers to dissect the intricate architecture of neural networks, providing insights into brain function and connectivity.
Understanding PRV-Based Tracing
Our PRV strains, derived from the less virulent Bartha strain, are engineered for strict retrograde labeling across multiple synapses. This allows for the detailed mapping of neural networks, identifying direct and indirect projections to targeted brain regions. Upon infecting neurons, the virus replicates and expresses the inserted gene, enabling the tracing of neural pathwa
ys back to their origins.
Figure 2. Injecting PRV into the brain area TU can mark the input neural network of different levels in the injection area.
Applications of PRV Tracing
Braincase's PRV tracing tools are invaluable for a variety of research applications, including:
Mapping Brain-Wide Input Networks: Identify multi-level input networks to specific brain regions.
Studying Developmental Neural Networks: Analyze changes in neural networks during neural development.
Investigating Neuropsychiatric Disease Models: Examine structural characteristics and alterations in neural networks associated with neuropsychiatric conditions.
Exploring Neural Injury and Rehabilitation: Assess the extent of neural network damage and recovery.
Tracing Peripheral to Central Nervous System Pathways: Map connections between peripheral organs and the central nervous system.
Featured Case Studies
Dorsal Striatum Network Tracing: Utilizing PRV152-GFP, we demonstrated the retrograde cross-multilevel tracing from the dorsal striatum, revealing connections to regions like R-NG, DMV, NTS, and others.
Figure 3. Validation of R-NG→PBNdl→SNc pathway (HanW, et al., Cell, 2018)
Bladder Wall Nerve Tracing: Injection of PRV-EGFP and PRV-RFP into the bladder walls showed retrograde tracing to cortical S1/M1L5 pyramidal neurons, indicating their role in bladder wall innervation.
Figure 4. Identification of the M1L5 pyramidal neuron population in the upper bladder cortex (YaoJ, et al., NatNeurosc, 2018)
Olfactory Bulb Input Network Analysis: Co-injection of PRV152(GFP) and PRV614(RFP) into olfactory bulbs revealed co-labeled neurons in the locus coeruleus, illustrating shared upstream networks.
Figure 5. Expression distribution of fluorescent proteins in different brain regions
Braincase's PRV Product Offerings
Our PRV tools come equipped with various fluorescent markers for easy visualization of traced pathways:
BC-PRV-531 PRV-CAG-EGFP: Offers green fluorescent polysynaptic retrograde tracing.
BC-PRV-724 PRV-CAG-mRFP: Provides red fluorescent polysynaptic retrograde tracing.
Explore Neural Networks with Braincase's PRV Tools Leverage Braincase's PRV-based tracing solutions to explore the depths of neural connectivity and function. Our tools are designed to facilitate groundbreaking discoveries in neuroscience research. Contact us for more information on our products and how we can support your research endeavors.
Keywords/Tags
Pseudorabies Virus, PRV, Polysynaptic Retrograde Tracing, Neural Circuit Mapping, Brain Function Analysis, Neuroscience Research