Exploring Brain Circuits with Advanced Neuronal Tracing Technologies
Introduction
At BrainVTA, we understand the intricate complexities of neuronal connectivity and its pivotal role in the brain's functionality. Our cutting-edge neuronal tracing tools are designed to provide unparalleled insights into brain circuits, overcoming the limitations of conventional tracers and leveraging the power of recombinant viral vectors for precise, cell-specific neuronal mapping.
Conventional Tracers vs. Viral Vector Tracing
Conventional tracers like CTB, HRP, and PHA-L have served as foundational tools in neuroscience. However, their limitations in cell specificity, efficiency, and multi-synaptic circuit mapping have led to the adoption of genetically engineered recombinant viral vectors. These vectors offer enhanced efficiency, specificity, and the ability to visualize complex neural connectivity across multiple synaptic steps.
Our Viral Vector Offerings
BrainVTA provides a comprehensive suite of viral vectors for neuronal tracing, including AAV, VSV, PRV, RABV, and HSV. Each vector is selected for its unique properties in tracing neural circuits, whether for monosynaptic, multisynaptic, anterograde, or retrograde tracing.
Table 1: Recombinant Viral Vectors for Neural Circuit Tracing
Type
Virus name
Classification
Genomic type
Non- transsynaptic
Adeno-associated virus, AAV
Parvoviridae
Single stranded DNA
Canine adenovirus, CAV
Adenovirus
Double strands DNA
Semliki Forest virus, SFV
Togaviridae
+ Single stranded RNA
Rabies virus (Glycoprotein G-deleted), RV-∆G
Rhabdoviridae
-Single stranded RNA
Herpes simplex virus amplicon, HSV amplicon
Herpesviridae
Double strands DNA
Trans-synaptic
Trans multisynaptic
Anterograde,multisynaptic
Herpes simplex virus, HSV H129
Herpesviridae
Double-stranded DNA
Vesicularstomatitis virus, VSV
Rhabdoviridae
-Single stranded RNA
Retrograde, multisynaptic
Pseudorabies virus, PRV
Herpesviridae
Double-stranded RNA
Rabies virus,RV WT
Rhabdoviridae
-Single stranded RNA
Trans-monosynaptic
Anterograde, monosynaptic
Herpes simplex virus (TK-deleted), HSV-∆TK
Herpesviridae
Double-stranded DNA
Adeno-associated virus,serotype 1, AAV1
Parvoviridae
Single stranded DNA
Retrograde, monosynaptic
Rabies virus, RV-∆G-EnvA
Rhabdoviridae
-Single stranded RNA
Pseudorabies virus (TK-deleted), PRV-∆TK
Herpesviridae
Double-stranded RNA
Advantages of Our Services
Efficiency: Our tools are designed for fast and high-efficient tracing, enabling rapid mapping of neural circuits.
Expertise: With a strong background in neuronal tracing, our team brings valuable insights and expertise to your research.
Safety: We prioritize hypotoxicity to ensure the well-being of neural tissues, coupled with strong signal clarity for precise imaging.
Affordability: Our competitive pricing ensures access to top-tier neuronal tracing tools without compromising on quality.
Custom Solutions and Support
Don't see exactly what you need? BrainVTA is committed to providing tailored solutions to fit your unique research requirements. Whether it's customizing viral vectors or addressing specific tracing challenges, our experienced scientists are here to guide you through every step.