BrainVTA is at the forefront of molecular cloning, offering a comprehensive suite of services to facilitate the creation of custom DNA constructs tailored to your research needs. Leveraging our advanced technical expertise and state-of-the-art facilities, we ensure the delivery of high-precision constructs, saving you valuable time and resources in the lab.
Custom Construct Creation
Our streamlined process for building your custom construct involves several key steps, from vector selection to the final packaging of viral particles. Our approach guarantees efficiency and accuracy at every stage.
Step 1: Vector Selection
Choosing the right vector is crucial for the success of your project. Our experts are here to guide you through the selection process, ensuring optimal compatibility with your research objectives.
Vector Comparison
Viral vector
AAV
LV
Ad
Genome
ssDNA
ssRNA (+)
dsDNA
Coat
Naked
Enveloped
Naked
Type
Non-integrating
Integrating
Non-integrating
Infection
Dividing and non-dividing cells
Dividing and non-dividing cells
Dividing and non-dividing cells
Packaging Capacity
4.9kb
6kb
7.5kb
Transgene expression
Potentially long-lasting
Long-lasting
Transient
Immune Response
Very Low
Low
High
Titer
Up to 1012-13v.g/ml
Up to 109TU/ml
Up to 1012pfu/ml
Expression abundance
High-level expression
Moderate to high level expression
High-level expression
Safety
Has been approved by the EU and FDA.
It has been used in CAR-T therapy in the human.
May cause some coughing and runny nose.
No pathogenicity has been found yet.
No pathogenicity has been found yet.
Used as a carrier for gene therapy drugs.
Step 2: Promoter Selection
We offer a variety of tissue-specific promoters to drive the expression of your gene of interest. Contact us if you don't see your desired promoter listed.
Step 3: ORF Addition
Add an Open Reading Frame (ORF) to your construct with ease. Our library includes a wide range of ORFs compatible with various research needs.
Step 4: Customization Options
Choose from tags, reporter linkers, or inducible systems to further customize your vector to meet the specific requirements of your study.