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Shanghai Model Organisms Center, Inc.
view supplier detailsCredit card payments now incur a 3% fee.
Shanghai Model Organisms Center, Inc.
view supplier detailsCredit card payments now incur a 3% fee.
| Mfr.No. | BHS19000004 |
| Service Type | Animal Model |
| Service Specification | Conditional Knockout (CKO) |
| Provider | GenoBioTX(SMOC) |
| Provider Location | Sugar Land, Texas, USA |
| Description |
IntroductionThe KO first (Conditional Ready) mouse model represents a groundbreaking advancement in genetic research. It offers a versatile approach to studying gene functions, enabling scientists to dissect complex genetic pathways with precision. Thismodel serves as a key tool in understanding gene expression and regulation in mammalian systems. The KO first (Conditional Ready) model employs a strategic arrangement of LoxP sites, flanking a target gene fragment, and an SA-IRES-reporter fragment, bordered by FRT sites. This unique configuration offers two distinct paths for genetic research:
 A schematic diagram representing the process of genetic modification using Cre-Lox and Flp-FRT recombination systems. KO First Allele: This represents the targeted gene with various elements inserted:
Cre Recombination: The Cre enzyme recognizes the LoxP sites and excises the DNA between them, resulting in the 'Reporter (KO) allele'. This allele retains the SA-IRES-LacZ-polyA sequence but loses the Neo cassette. Flp Recombination: The Flp enzyme recognizes the FRT sites and excises the DNA between them, resulting in a 'Floxed allele'. This allele has LoxP sites around the second exon (as marked by a '2' blue box), which can be later removed by Cre recombinase. KO Allele: Finally, Cre recombinase acts on the Floxed allele to remove the second exon, resulting in a KO allele, where the '2' box is missing, indicating that the exon has been successfully knocked out. The colors and shapes (blue boxes, green triangles, purple diamonds) are used to represent coding and non-coding regions of the gene, and the recombination sites, respectively. ApplicationsTracing Gene Expression:Understand where and when specific genes are active in living organisms. Essential in developmental biology and disease modeling. Conditional Knockout Models: Study gene function in specific tissues or at particular development stages. Crucial for researching complex diseases like cancer or neurological disorders. |
| Keywords/Tags | Genetic Engineering,Cre-Lox Recombination,Flp-FRT Recombination,Knockout (KO) Allele |
| Turnaround Time | 4-6 months, 6-9 months, 9-12 months |
