Targeted Conditional Overexpression, Random Transgene
Provider
GenoBioTX(SMOC)
Provider Location
Sugar Land, Texas, USA
Description
Introduction
Unlock the full potential of genetic research with SMOC's advanced genetic engineering techniques. Our targeted conditional overexpression using the Rosa26 locus and the efficient PiggyBac transposon system offer unparalleled precision and stability for your gene expression studies.
Targeted Conditional Overexpression Using Rosa26 Locus
The Rosa26 Locus - A Gateway to Precision
Explore the Rosa26 locus, a genomic safe harbor known for its consistent expression across various tissues without affecting the host's phenotype. Our Cre-LoxP system strategically inserts a recombinant vector into this locus, containing your gene of interest flanked by loxP sites for precise spatial expression.
Advantages of Rosa26 Targeting
Ensures stable gene expression
Allows large DNA fragment insertions (20-30kb)
Avoids random integration issues
Suitable for overexpression, gene function rescue, and modeling disease mutations
Rosa26 Knock-in Illustration
Visualize the precision of inserting a gene into the Rosa26 locus, from the initial wild type allele to the expression post-Cre recombinase action, ensuring accurate and targeted genetic manipulation.
Random Transgenes Using PiggyBac Transposon System
Swift and Efficient Genetic Integration
The PiggyBac transposon system is your fast track to generating transgenic mice. This system inserts your target gene into the mouse genome at specific TTAA sites, promoting transcriptionally active gene expression.
Benefits of PiggyBac Transposition
Rapid founder mice establishment
Increased likelihood of active gene expression
Quick verification of gene expression with luciferase reporters
PiggyBac Transposition Diagram
Understand the "cut and paste" actions of the piggyBac transposase with our detailed diagrams, demonstrating the seamless integration of genes into the mouse genome.
Whether you aim for targeted conditional overexpression or rapid transgene integration, SMOC provides the tools and expertise to advance your research. Explore our range of transgenic mouse models or collaborate with us to design a custom model that meets your exact specifications.
Ready to advance your genetic research with precision-engineered mouse models? Contact our expert team or Browse our catalog today to find the perfect solution for your study.