Gene Knockout Mouse Models by SMOC

Introduction:

• Discover the power of gene knockout mouse models in revealing critical genetic functions and cellular mechanisms.
• Deepen your knowledge about the origins of human diseases and streamline the process of evaluating drug candidates.
• Our offerings include an array of knockout models:
  • Standard Gene Knockout
  • Conditional Gene Knockout
  • KO First Approach
• Explore over 6000 ready-to-use genetically engineered mouse models or collaborate with our experts to tailor a model that precisely meets your research requirements.

Conventional (Constitutive) Gene Knockout

• Craft a standard gene knockout mouse model to permanently disable your target gene.
• Typical development time via CRISPR gene editing: 4-6 months.

• Process involves co-injecting synthetic sgRNAs and Cas9 mRNA into fertilized mouse eggs. The sgRNAs direct the Cas9 enzyme to specific genome locations, leading to target gene inactivation through the non-homologous end joining (NHEJ) repair mechanism.
• Traditional ES cell targeting method takes 9-12 months. It involves replacing one or more exons of the target gene with a neomycin resistance gene in embryonic stem cells, followed by breeding to produce homozygous knockout mice.

Conditional Gene Knockout

• Achieve temporal or spatial gene inactivation with our conditional knockout models, enhancing the precision of your research.
• Utilizes site-specific recombinase systems like Cre-LoxP and FLP-Frt for specific gene inactivation, with Cre-LoxP being the most common.
• Cre-LoxP system involves flanking a DNA sequence (to be deleted) with LoxP sites to create Flox mice, which are then crossed with Cre-expressing mice for targeted gene knockout.

• Production time via CRISPR: 6-9 months; via ES cell targeting: 9-12 months.

KO first

1. When mated with Cre-expressing mice, the deletion of the Neo gene and Flox region occurs, leading to reporter gene expression concurrent with the loss of the target gene.
2. Mating with Flp-expressing mice deletes the reporter fragment, creating conventional Flox mice, which can then be crossed with various Cre-expressing mice for diverse conditional knockout models.

Comparison