AAVnerGene AAV Capsid Library Construction Services

Mfr.No.

BHS17900004

Service Type

Gene Delivery

Service Specification

AAV, AAV Capsid Library

Provider

AAVnerGene

Provider Location

Rockville, Maryland, USA

Description

AAVnerGene AAV Capsid Library Construction Services

AAVnerGene provides specialized AAV capsid library construction services with two distinct library types based on our advanced ATHENA platforms. These services are designed to assist researchers in selecting the most efficient AAV capsid variants for their specific applications, whether they are established serotypes or novel capsid designs.

AAV Capsid Library(I) - ATHENA I Platform

The AAV Capsid Library(I) is built on our ATHENA I platform. This library is a collection of known AAV capsid variants, systematically organized to compare and select the best AAV serotypes or variants for specific applications. In this library, each capsid variant is linked to three different DNA barcoded genomes, all carrying the same reporter gene. The unique barcode is strategically placed between the coding sequence and the Poly-A signal. During AAV production, one capsid and its three corresponding barcode plasmids are co-transfected to produce a specific capsid AAV vector. All vectors are harvested, purified, and titered, either pooled or separately. Using Next-Generation Sequencing (NGS) targeting the barcodes (BCs), researchers can accurately assess which AAV capsid variant demonstrates optimal efficiency.

AAV Capsid Library(II) - ATHENA II Platform

The AAV Capsid Library(II), developed on our ATHENA II platform, is a random peptide insert library aimed at evolving novel AAV capsids with tissue-specific tropism. In this library, random peptide sequences are inserted into various sites of the AAV capsid protein. These peptides can modify the capsid's properties, such as tropism, transduction efficiency, and immunogenicity, potentially enhancing its utility for gene therapy applications. Through library screening, researchers can identify new capsid variants with improved characteristics and select the most suitable ones for their therapeutic needs.

Feature	AAV Capsid Library(I)	AAV Capsid Library(II)
Library Type	ATHENA I: Known AAV capsid collection library	ATHENA II: Random peptide insert library
Purpose	Evaluate different AAV capsids from known serotypes/variants	Create and evolve novel AAV capsids with specific tissue tropism
Complexity	Low (~1000 different AAV variants)	High (>1e9 variants)
Reporter	CAG-EGFP	CAG/P40-Cap
DNA Barcodes	One capsid corresponding to 3 different specific DNA barcodes	Random peptides themselves serve as barcodes
Production Condition	Triple-plasmid transfection as usual	Low copy number of library plasmid plus normal pHelper and pRep plasmid
Selection Method	NGS (~100M)	NGS (usually >100M)
Premade Library	Includes Common, Lung, BBB, etc.	Covers AAV1, AAV2, AAV3B, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh.10, AAVrh74
Library Construction Services	Yes	Yes
Selection Services	Yes	Yes
Prices	Based on request	Based on request

Keywords/Tags

AAV Capsid Library,DNA Barcodes,Library Construction Services,

Geographic Availability

Global

Technology Used	advanced ATHENA platforms

Payment Terms	Net 30
Additional Costs	shipping
Currency Accepted	USD
Refund Policy	non refundable

Certifications	RUO
Quality Control Steps	QC report, Validation
Safety Protocols	Biosafety Level
Support Availability	8/5, Business Hours
Customer Reviews or Ratings	4.5-star
Support Channels	Email, Chat
FAQ Section	Yes
Response Time	Within 24 hours
Handling Capabilities	Biohazard Level 2, Biohazard Level 1, Cold Chain, Sterile
Packaging Options	Plastic tubes
Customization Options	Protocol modification
Languages Supported	English,Mandarin
Collaboration Options	Remote

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AAVnerGene AAV Capsid Library Construction Services

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