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Home / Secondary Antibodies

Phycoerythrin Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

BHA10908728
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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
Request More Infos
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Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.0 mg/ml (by UV absorbance at 280 nm).
OD566nm/OD280nm ratio: 2.0
Description Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free
Emission Wavelength: 575 nm
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Absorption Wavelength: 488 nm
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Storage Store vial at 4° C prior to opening. DO NOT FREEZE. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date for properly stored material is six (6) months from date of opening.
Storage Condition -20C
Description The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Dilution

FACS1:100-1:250
SKU Supplier No. Size Your price Quantity
BHA10908728 SP005 250 ug $382.50
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