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Home / Secondary Antibodies

Peroxidase Conjugated IgG fraction of anti-Goat IgG [H&L] [Mouse]

BHA10910831
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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
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Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
Description Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Purity This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Mouse Serum, Goat IgG and Goat Serum.
Storage Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.
Storage Condition -20C
Description This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:6,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Dilution

ELISA1:2,000-1:6,000
SKU Supplier No. Size Your price Quantity
BHA10910831 SG016 5.0 mg $217.50
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