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Home / Secondary Antibodies

Peroxidase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

BHA10908240
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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
Request More Infos
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Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Description Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against or Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Storage This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Storage Condition -20C
Description This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:80,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Dilution

ELISA1:20,000-1:80,000
Reference
  • Sun, Z et al. "Downregulation of NPM expression by Her-2 reduces resistance of gastric cancer to oxaliplatin." Oncology Letters 4:2377-2384 (2017). DOI: 10.3892/ol.2017.5722. PubMed: 28454407 . Application: WB.
  • Wong, JC;Hasan, MR;Rahman, M;Yu, AC;Chan, SK;Schaeffer, DF;Kennecke, HF;Lim, HJ;Owen, D;Tai, IT;, et al. "Nucleophosmin 1, upregulated in adenomas and cancers of the colon, inhibits p53-mediated cellular senescence." Int. J. Cancer 133-7:1567-77 (2013). PubMed: 23536448.
SKU Supplier No. Size Your price Quantity
BHA10908240 SH026 250 ug $187.50
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