0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration
2.0 mg/ml (by UV absorbance at 280 nm)
Description
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease freeCaution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Storage
This product is stable for several weeks at 4° C as an undiluted liquid. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Storage Condition
-20C
Description
ELISA 1:180,000WESTERN BLOT 1:2,000 - 1:10,000IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000OTHER APPLICATIONS User OptimizedSuitable for immunoblotting (weste or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Dilution
ELISA
1:180,000
WB
1:2,000-1:10,000
IHC
1:1,000-1:5,000
Reviews of Peroxidase Conjugated Affinity Purified anti-Human IgG [H&L] [Rabbit]