0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration
10.0 mg/ml (by UV absorbance at 280 nm)
Description
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Purity
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
Storage
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of restoration.
Storage Condition
-20C
Description
ELISA 1:70,000 - 1:300,000WESTERN BLOT 1:5,000 - 1:25,000IMMUNOHISTOCHEMISTRY 1:1,000 - 1:5,000OTHER APPLICATIONS User OptimizedSuitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity.
Dilution
ELISA
1:70,000-1:300,000
WB
1:5,000-1:25,000
IHC
1:1,000-1:5,000
Reviews of IgG Fraction of anti-Rabbit IgG [H&L] [Goat]