Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins
Immunogen
Rabbit IgG, whole molecule
Quantity
250 µg
Reactivity
Rabbit
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Concentration
1.0 mg/ml (by UV absorbance at 280 nm)
Description
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease freeEmission Wavelength: 570 nmCaution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.Absorption Wavelength: 550 nm
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Storage
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Storage Condition
-20C
Description
FLOW CYTOMETRY 1:200 - 1:400IF MICROSCOPY 1:100 - 1:1,000OTHER APPLICATIONS User OptimizedPre-Conjugation ELISA DataMinimum ELISA Endpoint Dilution: 1:10,000 Actual: 1:41,000 Disposition: PASSSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Dilution
FACS
1:200-1:400
IF
1:100-1:1,000
Reviews of Cy3 Conjugated Affinity Purified anti-Rabbit IgG (H&L) [Goat]