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Home / Secondary Antibodies

Alkaline Phosphatase Conjugated Affinity Purified anti-Human IgG [H&L] [Donkey]

BHA10908511
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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
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Buffer 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Description Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.,This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.
Storage Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Storage Condition -20C
Description ELISA 1:2,000 - 1:10,000
WESTERN BLOT 1:500 - 1:2,500
IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000

Suitable for immunoblotting (weste or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Dilution

ELISA1:2,000-1:10,000
WB1:500-1:2,500
IHC1:200-1:1,000
SKU Supplier No. Size Your price Quantity
BHA10908511 SA019 250 ug $217.50
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